This learning activity explores infection prevention in healthcare settings from the perspective of non-clinical ancillary staff, covering the chain of infection, transmission-based precautions, correct PPE use, and role-specific responsibilities for environmental services, patient transport, dietary, and administrative personnel. Learners will examine how daily work tasks in each of these roles directly impact patient safety, how emerging verification and disinfection technologies support quality improvement efforts, and how a speak-up safety culture connects individual actions to facility-wide infection control outcomes.